1. INTRODUCTION:
Taxim-of is a synthetic
chemotherapeutic antibiotic of the flouroaninolone drug class considered to be
a second generation flouro auinolone. It is a combination of cefixime and
ofloxacin. These two components contain oxygen and nitrogen atoms. Therefore
they form complexes with transition metal ions.
Cefixime is an oral third
generation cephalosporin antibiotic. It is used to treat sinusitis,
tonsillitis, bronchitis, pneumonia, kidney infections and gonorrhea, oflaxacin
is a racemic mixture which consists of 50% levofloxacin and 50% of its “mirror
image”. Ofloxacin is a broad spectrum antibiotic that is active against both
gram positive and gram negative bacteria. It is limited to the treatment of
proven serious and life threatening bacterial infections 2-3.
2. EXPERIMENTAL SOLUTION:
One ml each of cobalt nitrate [1 x
10–3] and 1 mg/ml Taxim-of are taken in a 10ml standard flask. The
solution is made up to the mark with a buffer solution of required pH and
shaken well for uniform concentration. Similarly a blank solution is formed
without the drug. The absorption spectrum is recorded in the wavelength a
region of 400-750nm wavelength range.
3. RESULTS AND DISCUSSION:
3.1 EFFECT
OF PH
The effect of pH on the
complexation is studied. The absorbance values increase from pH–1 to
pH–10, the colour and λmax
are observed that Co(II) forms a pink coloured complex in slightly acidic
medium. Therefore a solution of pH-6 is chosen for further studies due to the
stability. The maximum absorbance is
noticed at 620nm. The data is presented in table-1.
Absorption Spectra of Cobalt(II)
ion at different pH values
[Co(II)] = 1 x 10Żł M [Taxim-of] = 1 mg/ml
Wavelength
3.2 EFFECT
OF COBALT (II) ION CONCENTRATION
The effect of Co(II) ion concentration on the absorbance is
studied. The concentration of metal ion is increased. The concentration of
Taxim-of is kept constant at 1 mg/gl. The pH of the solution is maintained at
6. From the result it may be concluded that Co(II) can be determined in the
range of 0.73 – 5.15 µg/25ml.
Concentration of Co(II) Ion [1 x 10-3 M]
3.3 EFFECT
OF DRUG CONCENTRATION
The effect of Taxim-of
concentration is studied in the range of 1 to 6 mg/ml. The absorbance values
are measured at 620nm. The concentration of
Co(II) is fixed 1 x 10–3 M. Using the present method the drug
can be determined in the range of 1 to 6 mg/ml.
Concentration of drug [Taxim-of] mg/ml
3.4 EFFECT
OF TIME ON THE REACTION
The effect of time on absorbance is
studied for a period of one hour for the same solution at regular interval of
ten minutes. It is found that the absorbance values remain constant.
3.5 EFFECT
OF THE ORGANIC SOLVENTS
Organic solvents generally
influence a complexation reaction. Therefore effect of methanol, acetone,
propanol, acetonitrile 50% by volume are investigated. An analysis of data
reveals that the absorbance is not much when methanol, propanol or acetone were
used. However in the presence of acetonitrile there is a decreases in
absorbance which is unfavorable for the complex formation.
Effect or organic solvents on
absorbance
pH=6 [Co(II)] = 1 x 10-3 M [Taxim-of]=
1 mg/ml λmax = 450nm
|
S.No |
Organic solvent |
Absorbance |
|
1 |
No solvent |
0.20 |
|
2 |
Methanol |
0.21 |
|
3 |
Acetone |
0.19 |
|
4 |
Propanol |
0.20 |
|
5 |
Acetonitrile |
0.12 |
4. COMPOSITION OF THE COMPLEX:
The stoichiometry of the Co(II) and
Taxim-of complex is determined by Job’s method of continuous variation. In the
Job’s method a series of solutions containing varying volumes metal ion and
Taxim-of solution of required concentration were taken in 10ml volumetric
flask. The absorbance values of these solutions were recorded in each case and
it is clear from the figure that Co(II)
forms 1:1 complex with reagent.
Absorbance
Volume of the Co(II) metal / drug ml
5. EFFECT OF DIVERSE IONS:
The effect of interfering ion on
the determination of Co(II) was investigated by adding known concentration of
various anions and cations was determined. The tolerance limits of various ions
are presented in table.1
Table 1 Tolerance limit of Foreign
Ions
|
Foreign Anions |
Tolerance limit µg/ml |
Foreign Cations |
Tolerance limit µg/ml |
|
Thiosulphate |
15.5 |
Fe (III) |
4.68 |
|
Oxalate |
8.85 |
Cr (VI) |
5.18 |
|
Nitrate |
130.53 |
Se (IV) |
5.45 |
|
Iodide |
253.80 |
Pd (II) |
0.12 |
|
Chloride |
54.62 |
Cu (II) |
0.45 |
|
Fluoride |
20.54 |
Ni (II) |
0.612 |
|
Acetate |
43.70 |
Ti (IV) |
6.96 |
|
EDTA |
1667 |
Cd (II) |
0.804 |
|
|
|
Ru (III) |
13.26 |
|
|
|
Mo (VI) |
19.2 |
|
|
|
Sn (II) |
14.84 |
|
|
|
Zr (IV) |
10.73 |
|
|
|
Sr (II) |
12.75 |
|
|
|
Al (III) |
13.49 |
|
|
|
Mn (II) |
19.98 |
|
|
|
Mg (II) |
32.41 |
|
|
|
U (VI) |
82.80 |
|
|
|
W (VI) |
63.95 |
|
|
|
La (III) |
52.91 |
|
|
|
Th (IV) |
64.01 |
6. CONCLUSION:
The proposed procedure is simple,
sensitive and rapid it is possible to determine the metal ion and Taxim-of in
the range of 0.73 – 5.15 µg/25ml and 1 to6 mg/ml respectively.
7. APPENDIX:
Simple, selective spectrophotometric
methods are developed for the determination of Taxim-of. The procedure is based
on the observation that Taxim-of forms coloured complex with Co(II). The
proposed methods can be employed for the analytical determination of metalion,
in the range of micrograms. The method is successfully applied for the
determination of Taxim-of in pharmaceutical formulation.
8. REFERENCES:
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M.S. (2008) Spectrochimica Acta part-A, Molecular and Bio molecular spectroscopy
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J.R., “Annals of Internal Medicines” 85(5); 654 – 6 (1975).
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Demanet J.C., “Correction of antidiuresis by minocycline”. 294 (18): 1975.
5. Tolstoi L.G., “Medcap
Pharmacotherapy 4(1), 2002.
6. Lochr, G.W.
“Minocycline and Pharmacogentios and preventive Medicine”, George thineme,
Switzerland (1966).
7. Sandell, E.B. “Colorimetric
determination of trans of metals”, Inter science, New York, 367 (1950).
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and Shah Alam, MD (2003) Spectroscopy 17, 45-52.
9. Job, Pann.chem 9, 113
(1928).
10. Yoe, J.H. and Jones,
A.L. (1944) Ind. Engg. Chem. Anal Ed. 16,111-5.
Received on 06.12.2013 Modified on 25.12.2013
Accepted on 14.01.2014 © AJRC All right reserved
Asian J. Research Chem. 7(3): March 2014; Page 275-277